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Question
Why are rna and dna−rna hybrids denser than double-stranded dna?
Answer
Ribonucleic acid, the basic unit of RNA, has a hydroxyl (-OH) on the second carbon atom. On the other hand, the deoxyribonucleic acid, the basic unit of DNA, has a single hydrogen in the second carbon. This chemical structure difference makes RNA heavier than DNA due to the extra carbon atom. The RNA/RNA-DNA hybrids can be separated from double-standard DNA using a high-resolution centrifuge due to this difference in weight. The image belows shows the extra oxygen atom in the 2′ carbon
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